hydrogen peroxide (peroxide), tseem hu ua hydrogen peroxide, hydrogen dioxide, yog ib hom tshuaj tua kab mob peroxide, yog cov khoom lag luam ntawm qee cov tshuaj txo qis thiab oxidation nyob rau hauv qhov, tsis tshua muaj, tsuas muaj kab nyob hauv qee cov nroj tsuag SAP thiab los nag thiab daus. Cov mis molecular ntawm hydrogen peroxide yog H2O2 thiab qhov hnyav molecular yog 34.015. Oxygen atoms coj tsis sib npaug sp3 hybrid orbitals rau daim ntawv cog lus, cov molecules yog covalent polar molecules, thiab cov qauv peb sab zoo li ob nplooj ntawv ntawm ib nrab-qhib phau ntawv.
Hydrogen peroxide yog ib qho muaj zog oxidizing tus neeg sawv cev, tsis muaj zog acidic, tsis muaj xim thiab tsis muaj ntxhiab pob tshab kua. Ntev npaum li qhov chaw cia khoom zoo, tsis muaj qhov tsis huv, cov ntshiab hydrogen peroxide yog qhov ruaj khov, tuaj yeem khaws cia rau lub sijhawm ntev thiab tsis tshua muaj decomposed, tab sis thaum rhuab mus rau 153 ° C lossis siab dua kub yuav ua rau tawg tawg. Lub decomposition tus nqi ntawm hydrogen peroxide nyob rau hauv alkaline nruab nrab yog sai npaum li cas nyob rau hauv acidic nruab nrab, thiab cov yam tseem ceeb cuam tshuam rau nws stability yog impurities (ntau cov hlau hnyav xws li: Fe2+, Mn2+, Cu 2+, Cr3+), pH tus nqi, qhov kub thiab txias, lub teeb, thiab qhov roughness ntawm sab hauv ntawm lub thawv ntim khoom. High concentration ntawm hydrogen peroxide tuaj yeem ua rau ntau cov kuab tshuaj organic hlawv thiab hnov mob nrog manganese dioxide kom tawg. Yog li ntawd, hydrogen peroxide yuav tsum tau muab tso rau hauv lub thawv yas kaw, khaws cia rau hauv qhov chaw txias, txwv tsis pub nyob rau hauv lub hnub raug, thiab tsis txhob muaj kuab paug thiab ua nruj ua tsiv.
Hydrogen peroxide yog cov tshuaj tua kab mob uas muaj txiaj ntsig zoo, thiab qee qhov kev daws teeb meem ntawm hydrogen peroxide tuaj yeem tua cov kab mob xws li cov kab mob propagans, spores, fungi thiab kab mob.
Ib
Action mechanism
1. Hydrogen peroxide decomposes nyob rau hauv catalysis ntawm photochemistry, ionizing hluav taws xob, hnyav hlau thiab converting hlau ions los tsim ntau yam chemical pawg, xws li active oxygen hom thiab derivatives, OH pawg, thiab lwm yam, uas muaj zog tua cov kab mob. Los ntawm kev hloov pauv permeability barrier ntawm cov kab mob, cov protein enzymes, amino acids thiab nucleic acids ntawm cov kab mob raug rhuav tshem, ua rau cov kab mob tuag taus.
2. Hydrogen peroxide yog ib qho oxidizing tus neeg sawv cev uas tuaj yeem ionize cov molecules lossis atoms ntawm cov kab mob ntawm cov kab mob thiab ua rau cov lipid saw ntawm phab ntsa ntawm tes tawg, yog li rhuav tshem cov phab ntsa ntawm tes.
3. Hydrogen peroxide tuaj yeem oxidize Sh-muaj enzymes, ua rau poob ntawm metabolic activation enzyme muaj nuj nqi, ua rau cell division thiab kev loj hlob tsis zoo.
4. Tom qab nkag mus rau cov kab mob, cov tshuaj lom OH pawg tsim tawm, uas ua rau phosphodiester daim ntawv cog lus ntawm DNA thiab ua rau nws tawg.
OB
Cov xwm txheej cuam tshuam rau kev tua kab mob
Cov kab mob bactericidal ntawm hydrogen peroxide yog cuam tshuam los ntawm lub sij hawm, concentration, pH nqi, txheeb ze humidity, organic tiv thaiv, lub cev thiab tshuaj yam tseem ceeb, thiab lwm yam.
2.1 Lub sijhawm ua haujlwm
Nrog rau ncua sij hawm, cov nyhuv bactericidal tau txhim kho. Thaum 10% hydrogen peroxide tau siv rau Bacillus subtilis dub variant spores, lub sijhawm kho ntev dua, ntau qhov spores tuag.
2.2 Concentration
Nrog rau qhov nce ntawm hydrogen peroxide concentration, cov nyhuv bactericidal tau txhim kho. Cov kev tshawb fawb tau pom tias nyob rau hauv qhov xwm txheej ntawm 20 degree, qhov sib txawv ntawm hydrogen peroxide tau siv los tua Bacillus subtilis dub variant spores. Cov txiaj ntsig tau pom tias qhov siab dua ntawm hydrogen peroxide, lub sijhawm ua kom tsis muaj menyuam. Txawm nyob rau hauv cov kua los yog gaseous daim ntawv, cov bactericidal nyhuv ntawm hydrogen peroxide yog zoo proportional rau nws concentration. Cov kev tshawb fawb tau ua pov thawj tias n tus nqi ntawm hydrogen peroxide tua Bacillus typhi (concentration coefficient lossis dilution coefficient, siv los qhia txog kev cuam tshuam ntawm cov tshuaj tua kab mob concentration ntawm cov tshuaj tua kab mob, qhov ntau dua tus nqi, qhov pom tseeb ntawm qhov kev hloov pauv ntawm cov tshuaj tua kab mob. ) yog 0.5, thiab cov dilution coefficient ntawm tua Bacillus subtilis spores yog 0.7~0.9. Zhang Wenfu et al. nqa tawm kev sim tua ntawm Bacillus subtilis dub variant spores ntawm qhov sib txawv ntawm hydrogen peroxide ntawm 20 degree. Cov txiaj ntsig tau pom tias qhov siab dua qhov siab dua, lub sijhawm tua luv dua, thiab kev sib raug zoo ntawm cov concentration thiab lub sijhawm ua haujlwm yog linearly tsis zoo (Y=2.944-1.134X, r =-0.989 , P <0.01).
Raws li cov qauv hauv qab no concentration coefficient: n =[(LGt2-LGT1)/(lgC1-lgC2)], thaum lub concentration ntawm hydrogen peroxide hloov ntawm 5% thiab 25%, nws cov concentration coefficient (n) yog 1.13. Raws li cov concentration coefficient, nyob rau hauv qhov ntau ntawm kev sim qhov tseem ceeb, qhov concentration ntawm hydrogen peroxide yog txo los ntawm ib nrab, thiab lub sij hawm yuav tsum tau ua yog ncua los ntawm 2.19 lub sij hawm.
2.3 Kub
Lwm yam kev mob tseem tsis tau hloov pauv, cov nyhuv bactericidal ntawm hydrogen peroxide kua yog txhim kho nrog qhov kub thiab txias. Thaum 10% hydrogen peroxide tau siv los tua Bacillus subtilis dub variant spores, cov tshuaj kub tau cuam tshuam tsis zoo nrog lub logarithm ntawm lub sijhawm yuav tsum tua cov kab mob spores. Ntawm 70 degree, 10% hydrogen peroxide tuaj yeem tua tag nrho cov spores hauv 1min xwb, thaum nyob ntawm 10 degree nws yuav siv sijhawm 205min kom ua tiav cov txiaj ntsig zoo ib yam.
2.4 txheeb ze humidity
Cov av noo nyob rau hauv cov huab cua tsawg dhau los yog siab dhau lawm, uas muaj kev cuam tshuam tsis zoo rau cov kab mob bactericidal ntawm hydrogen peroxide. Hauv kev sim tua cov kab mob staphylococcus albicans nrog cov tshuaj tua kab mob hauv huab cua raws li hydrogen peroxide, cov txiaj ntsig tau pom tias kev tua tus nqi yog 91.13% thaum cov txheeb ze av noo yog 52% ~ 54% rau 10min. Kev tua tus nqi yog 98.53% thaum tus txheeb ze av noo yog 72%-74%. Thaum tus txheeb ze av noo yog 90% ~ 92%, tus nqi tua yog 68.98%.
2.5 pH tus nqi
Txawm hais tias nws yog cov ntshiab hydrogen peroxide lossis hydrogen peroxide nrog rau lwm cov khoom xws li potassium iodide, lub peev xwm ntawm cov kab mob hauv acidic ib puag ncig muaj zog dua li hauv ib puag ncig alkaline.
2.6 Organic Matter
Kev tiv thaiv ntawm cov organic teeb meem muaj qee yam cuam tshuam rau cov kab mob bactericidal ntawm hydrogen peroxide. Thaum tua cov kab mob uas kis los ntawm cov ntshav, kua paug, hnoos qeev thiab tso zis, lub sijhawm ua haujlwm yuav tsum txuas ntxiv mus. Txawm li cas los xij, qee qhov kev tshawb fawb tau taw qhia tias cov kab mob bactericidal ntawm cov concentration ntawm hydrogen peroxide yog qhov tsawg heev, tshwj xeeb tshaj yog nyob rau hauv qhov kub thiab txias, qhov ua kom tsis muaj menyuam tsis pom tseeb.
2.7 Kev sib koom ua ke
Nyob rau hauv xyoo tas los no, cov txiaj ntsig tau pom tias lub teeb ultraviolet, hlau ions, tsis zoo ions thiab lwm yam physicochemical yam muaj txiaj ntsig zoo rau cov kab mob bactericidal ntawm hydrogen peroxide.
(1) Ultraviolet lub teeb muaj kev sib koom ua ke ntawm hydrogen peroxide sterilization. Cov hluav taws xob ultraviolet tuaj yeem txhawb kev decomposition ntawm hydrogen peroxide los tsim cov dawb radicals, uas ua rau cov spores protein thiab cortex, ua rau cov kab mob tuag sai. Cov txiaj ntsig tau pom tias kev tua tus nqi ntawm Bacillus subtilis dub variant yog 99.90% thaum 3% hydrogen peroxide thiab 70uw / cm2 ultraviolet lub teeb tau siv rau 5min, thaum tua tus nqi yog 17.72% thiab 99.20% thaum hydrogen peroxide thiab ultraviolet siv ib leeg. .
(2) Cov bactericidal synergistic nyhuv ntawm poov tshuaj iodide ntawm hydrogen peroxide tau raug pov thawj nyob rau hauv tsev thiab txawv teb chaws. Qhov sib xyaw ua ke ntawm 0.125% hydrogen peroxide thiab 0.125% potassium iodide rau 3min yog siab dua li ntawm 0.250% hydrogen peroxide thiab 0.250% potassium iodide rau tib lub sijhawm. Qhov ntau dua qhov sib koom ua ke coefficient, cov nyhuv muaj zog dua.
(3) Hydrogen peroxide yog tov nrog glutaraldehyde los tsim aldehyde peroxide, uas tuaj yeem txhim kho cov kab mob bactericidal. Cov txiaj ntsig tau pom tias thaum 5% hydrogen peroxide thiab 0.5% glutaraldehyde ua ke ua ke rau 10 ~ 30min, kev tua tus nqi ntawm Bacillus subtilis nigrospora yog li 99.99%, uas yog ntau dua li ntawm 60.12% thiab 88.18. % thaum siv hydrogen peroxide thiab glutaraldehyde ib leeg.
(4) Hydrogen peroxide yog koom tes nrog ultrasound. Nws tau raug tshaj tawm tias kev siv ultrasound siab thiab 6% hydrogen peroxide tuaj yeem tua Bacillus cereus thiab Candida albicans hauv 10min nkaus xwb, thaum 30min kho nrog ultrasound lossis hydrogen peroxide ib leeg tseem tsis txaus tua lawv.
(5) Hydrogen peroxide koom tes nrog plasma, thiab synergistic bactericidal nyhuv yog vim lub fact tias hydrogen peroxide yog yooj yim decomposed mus rau ntau yam dawb active pab pawg nyob rau hauv lub plasma xeev.
(6) Hydrogen peroxide yog koom tes nrog hlau ions (Fe2+, Cu2+, Ag+), thiab hlau ions kuj tseem tuaj yeem txhawb kev decomposition ntawm hydrogen peroxide los tsim cov dawb radicals thiab ua kom cov kab mob tuag taus.
(7) Kev sib koom ua ke ntawm hydrogen peroxide thiab surfactant tau pom tias qhov sib ntxiv ntawm surfactant D-33 lossis Sulfonol thaum lub sijhawm tshuaj tua kab mob ntawm hydrogen peroxide tuaj yeem nqaim txoj kab uas hla ntawm aerosol, yog li txhim kho kom tsis muaj menyuam.